By: Abdulaziz Yahya Algamdi
Abstract
Nine dichromate resistant bacteria were isolated: Bacillus cereus, Bacillus pumilus, Bacillus licheniformis, Bacillus subtilis, Acinetobacter radioresistens, Acinetobacter venetianus, Ochrobacterum sp, Massilianiabensis, and Leucobacter chromiireducens. All of the isolates absorbed and reduced potassium dichromate (Cr6+ ) from the growth medium amended by dichromate Cr6+ at concentration of 100 ppm at higher percentages in the presence of dehydrogenase NADH+ compared to bacteria growth in medium without dehydrogenase NADH+. Absorption of potassium dichromate Cr6+ from the growth medium by the bacteria increased with increasing incubation time over 96 hrs. 
The nine bacteria species isolated from the leather tanning factories in Riyadh were shown to produce both intracellular and extracellular chromium reductase, with the percentage of precipitate of the internal enzyme is always being higher than the external precipitate except in the case of A. radioresisten which showed values of 26% for the intracellular enzyme and 52% for extracellular enzyme.
Author Information:
Abdulaziz Yahya Algamdi, Sulaiman Ali Alharbi, Milton Wainwright, Samir G. Al-Solaimani
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
Department of Molecular Biology and Biotechnology, The University of Sheffield, England
Department of Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia
Key words: Detoxification, NADH+, Potassium dichromate (Cr+6), Chromium, Resistant bacteria.
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