Detection, susceptibility and molecular characterisation of ESBL- producing E. coli causing urinary tract infection – JBES

ecoli-bacteriaFereshteh Javadian, Zahra Sepehri, Hamideh Khaje, Raziyeh Farazmand, Zahra Miri5 , Naghmeh Gholipoura and Zahra Shahi

Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran

Zabol University of Medical Sciences, Zabol, Iran

Institute of Biotechnology Research, University of Zabol, Zabol, Iran

M. Sc. Student of Biochemistry, Payam Noor University of Mashhad, Mashhad, Iran

M. Sc. Biochemistry, Payam Noor University of Mashhad, Mashhad, Iran

Department of molecular genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran

Keyword: TEM gene, PCR, E. coli, ESBLs

Abstract

In this study we evaluate role of a rapid polymerase chain reaction (PCR) assay compared with traditional empiric therapy in extended spectrum b-lactamases production E. coli detection, using a literature-derived model. A cross-sectional study was performed. Sample were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2010- 2011. Ninety isolates of E. coli from urinary tract infection were collected, tested for antibiotics with disc diffusion method, minimum inhibitory concentration (MIC) for ceftazidime and resistant gene TEM were detected by PCR. E. ColiThe result showed forty out of ninety E. coli isolates were ESBLs producing organisms by disc diffusion. Antibiotic susceptibility of E. coli isolates was evaluated for 9 antimicrobial. However, overall, E. coli were resistance to 9 of the agent including ceftriaxone (100%), ceftazidime (100%) amoxicillin (100%), azithromycin (95%), cefixime (87.5%), tetracyclin (87.5%), erythromycin (100%). nalidixin acid (85%) and difloxcain (75%) respectively. The distribution of antibiotic-resistant TEM gene according to PCR was 30%. Totally 82.5% were MIC observed as ceftazidime-resistant. We conclude that the TEM gene PCR assay is a rapid, sensitive and clinically useful test, particularly for the early detection of ESBLs- producing E. coli.

jbes-vol5no1-p291-299Get the original articles in Source: Volume 5, Number 1, July 2014 – JBES

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

Related Post: Measuring the concentrations of heavy metals in dust in Ahvaz – Iran – JBES

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