Intra-specific variations of Rubus sp. (Rosaceae) in Northern Iran: morphometric analysis and microsatellite markers – JBES

Rubus,Ataei-E Jaliseh Somayeh, Mehregan Iraj, Tarang Alireza and Nejhadsattary Taher

Department of Biology, College of Science, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

Department of Genomics, Branch of North Region of Iran, Agricultural Biotechnology Research Institute of Iran (ABRII), Rasht, Iran

Key words: Iran, morphology, polyploidy, Rubus, simple sequence repeat marker.

Abstract

During the growing season, young raspberry leaf samples were collected from natural populations in northern Iran. A total of 65 Rubus samples were collected at altitudes ranging from 0 to 1021 meter above sea level. Morphometric and molecular studies were performed in seven Rubus species and four uncertain species from the sect. Rubus L. 7 species including R. caecius, R. sanctus, R, discolor, R. dolichocarpus, R. hyrcanus, R. persicus and R. hirtus are. In total, the delimitation of species of Rubus northern Iran, from 27 morphological characters and 13 SSR primer pairs were used. The dendrogram based on quantitative morphological traits, species are completely separated, and even species were differentiated on the basis of geographical pattern. RubusA total of 12 loci and 29 alleles were detected. The number of alleles observed for each locus ranged from 1 to 6, with an average of 3.08 alleles per locus. The SSRs achieved cumulative polymorphism information content (PIC) of 0.9444. These PIC values demonstrated that, in spite of the low number of primers used, the SSR were sufficiently polymorphic and informative. Expected heterozygosities (He) were 0.021 to 0.286. The dendrogram based on microsatellite markers, no clear geographic pattern to interpret the results, we do not see. Status is unknown species and genotypes with high affinity and proximity to each other show. In general, the limits of species, the occurrence of hybridization between species with different ploidy levels between Rubus genotypes, is not subject to any specific geographic pattern.

jbes-vol5no1-p189-198Get the original articles in Source: Volume 5, Number 1, July 2014 – JBES

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

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