Comparative analysis of some biochemical responses of winter and spring wheat cultivars under low temperature – IJAAR

triticum-aestivumAhmad Tahmasebi, Hassan Pakniyat

Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran

Key words: Low temperature, Triticum aestivum, Superoxide dismutase, Peroxidase, Catalase.

Abstract

To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Triticum aestivumRapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress.

ijaar-v7no4-p14-22Get the original articles in Source: Volume 7, Number 4, October 2015 – IJAAR

Journal Name: International Journal of Agronomy and Agricultural Research (IJAAR)

Published By: International Network for Natural Sciences

Related Post: Inheritance of stem rust (Puccinia graminis Pers. F. Sp. Tritici ericks and E. Hen) resistance in bread wheat (Triticum aestivum L.) lines to TTKST race – IJAAR

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