Antibacterial activity of important medicinal plants on human pathogenic bacteria – IJAAR

herb-garden_healthKhaista Rahman, Muhammad Nisar, Amin Ullah Jan, Muhammad Suliman, Ajmal Iqbal1, Ayaz Ahmad, Rukhsana Ghaffar

Department of Botany, University of Malakand, Chakdara Dir Lower, KPK, Pakistan

Department of Biotechnology, University of Malakand, Chakdara Dir Lower, KPK, Pakistan

Department of Pharmacy, University of Malakand, Chakdara Dir Lower, KPK, Pakistan

Key words: Antibacterial Activity, Medicinal Plants, Agar Well Diffusion Method.

Abstract

The resistance of pathogenic bacterial strains to antibiotics is the major burning issue around the world. To minimize the strain resistance to antibiotic local medicinal plant were selected. The leaves of medicinal plants (Ajuga bracteosa Benth, Calotropis procera and Zizyphus sativa Geartn) were collected and antibacterial activity was examined through agar well diffusion method. herbal-plantThe crude extracts were obtained by using methanol and n-hexane as the extraction solvent. Three concentrations (100 mg/ml, 50 mg/ml, and 25 mg/ml) were used to check the antibacterial activity of plant extracts. Each plant sample was tested against two Gram-positive (Bacillus cereus, Bacillus subtilis) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi) bacteria. The methanol extracts showed better activity against all the tested bacterial strains, except Zizyphus sativa Geartn showed inactive against Pseudomonas aeruginosa and Salmonella typhi. The nhexane extracts showed moderate activity against all the bacterial strains, whereas the n-hexane extract of Zizyphus sativa Geartn were inactive against Salmonella typhi and Bacillus cereus. It is concluded that methanol extract showed good antibacterial activity as compared to n-hexane extract and further study is needed to check the activity of the tested plants against the other bacterial strains.

ijaar-v6no6-p106-111Get the original articles in Source: Volume 6, Number 6, June 2015 – IJAAR

Journal Name: International Journal of Agronomy and Agricultural Research (IJAAR)

Published By: International Network for Natural Sciences

Related Post: Effect of nitrogen rates and weed interferenceon yield and yield components of corn (Zea mays L.) – IJAAR

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