Khaista Rahman, Muhammad Nisar, Amin Ullah Jan, Muhammad Suliman, Ajmal Iqbal1, Ayaz Ahmad, Rukhsana Ghaffar
Department of Botany, University of Malakand, Chakdara Dir Lower, KPK, Pakistan
Department of Biotechnology, University of Malakand, Chakdara Dir Lower, KPK, Pakistan
Department of Pharmacy, University of Malakand, Chakdara Dir Lower, KPK, Pakistan
Key words: Antibacterial Activity, Medicinal Plants, Agar Well Diffusion Method.
The resistance of pathogenic bacterial strains to antibiotics is the major burning issue around the world. To minimize the strain resistance to antibiotic local medicinal plant were selected. The leaves of medicinal plants (Ajuga bracteosa Benth, Calotropis procera and Zizyphus sativa Geartn) were collected and antibacterial activity was examined through agar well diffusion method. The crude extracts were obtained by using methanol and n-hexane as the extraction solvent. Three concentrations (100 mg/ml, 50 mg/ml, and 25 mg/ml) were used to check the antibacterial activity of plant extracts. Each plant sample was tested against two Gram-positive (Bacillus cereus, Bacillus subtilis) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi) bacteria. The methanol extracts showed better activity against all the tested bacterial strains, except Zizyphus sativa Geartn showed inactive against Pseudomonas aeruginosa and Salmonella typhi. The nhexane extracts showed moderate activity against all the bacterial strains, whereas the n-hexane extract of Zizyphus sativa Geartn were inactive against Salmonella typhi and Bacillus cereus. It is concluded that methanol extract showed good antibacterial activity as compared to n-hexane extract and further study is needed to check the activity of the tested plants against the other bacterial strains.
Get the original articles in Source: Volume 6, Number 6, June 2015 – IJAAR
Published By: International Network for Natural Sciences