S.T. Okiobé, M. Abossolo Angue, B.P. Bougnom, Boyomo Onana, D. Nwaga
Laboratory of Soil Microbiology, Biotechnology Centre, University of Yaounde I, P. O Box 812 Yaounde, Cameroon
Department of Microbiology, Faculty of Science, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon
Department of Earth Sciences, Faculty of Science, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon
Key words: Inoculum production, arbuscular mycorrhizal fungi, nutrient solution, soil texture.
Arbuscular Mycorrhizal Fungi (AMF) can increase the yield of plants from 50 to 200 %, but the large scale multiplication of AMF inocula is difficult because of their trophic nature, and this represents a big challenge in tropical areas. Within the framework of the study, two separate experiments were conducted in order to determine the most favorable conditions for AMF spore production. The effect of Rorison’s nutrient solution (0, 4 and 8 ml/l) was tested on 6 types of AMF strains for spores production, while in the second experiment; it was the effect of soil-sand mixture variation (5-57 % clay content) that was evaluated in AMF spores production. A local variety of Sorghum bicolor was used as the host plant during the trials. Experiments were performed in completely randomized design with 4 replicates. Results showed that Rorison’s nutrient solution and soil texture significantly (P<0.05) influenced plant growth, symbiotic and biochemical parameters. Nutrient solution induced significant increase in root colonization (5 to 36 %), and AMF spore production (12 to 23 spores/g of soil). The highest concentration of Rorison’s nutrient solution promoted more spore formation, but that was not translated in plant yield. Soil texture variation had a significant impact on AMF root colonization and spores production, since mixture of sand and clay, with clay variation from 20-43% was found to favor both parameters. These data suggest that soil texture variation and nutrient solution concentration can significantly improve AMF spores production and Sorghum symbiotic performances.
Get the original articles in Source: Volume 6, Number 5, May 2015 – IJAAR
Published By: International Network for Natural Sciences