Molecular taxonomy and phylogeny of Silene species (Caryophyllaceae) using DNA-based markers – JBES

silene-8Farrokh Ghahremaninejad, Abdolhamid Angaji, Maryam Etemad, Fatemeh Vahidynia, Farideh Attar

Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, 43 Dr. Mofatteh Avenue, Tehran, Iran

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, 43 Dr. Mofatteh Avenue, Tehran, Iran

Department of Botany, School of Biology, College of Sciences, Tehran University, Tehran, Iran

Key words: Silene, phylogeny, SRAP, AMP-PCR, MP-PCR, RAPD.

Abstract

DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. Silene is a genus of flowering plant in the family Caryophyllaceae. This study compared the phylogenetic relationship within and between 16 Silene sections, including 24 species (Silenoideae: Caryophyllaceae) silene-latifolia-male_hr-2using some molecular markers such as Random Amplification of Polymorphic DNA (RAPD), Microsatellite Primed PCR (MP-PCR), Anchored Microsatellite Primed PCR (AMP-PCR) andSequence-Related Amplified Polymorphism (SRAP), Phylogenetic analysis was carried out by parsimony, and neighbour joining model. Finally, our molecular analysis did not confirm morphological viewpoints completely.

jbes-vol4no4-p112-124Get the original articles in Source: Volume 4, Number 4, April 2014 – JBES

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

Related Post: Microbiological effects of high pressure processing on food — JBES

Download PDF

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s