Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.) – JBES

cuscuta-epithymum-16312Seyed Kamal Kazemitabar, Simin Azoush, Amin Sadeghi Alikelayeh

Plant Breeding and Biotechnology, Sari Agriculture Sciences and Natural Resources University (SANRU), Iran

Biotechnology, Sari Agriculture Sciences and Natural Resources University (SANRU), Iran

MSc Student of Biotechnology, Zabol University, Iran

Key words: Genetic diversity, weed dodder (Cuscuta epithymum L), RAPD, ISSR

Article published on April 02, 2014

Abstract

Weed dodder is a perennial herb belonging to the Convolvulaceae family and is widely used as a valuable herbal drug in traditional medicine. In this study, ISSR and RAPD markers were used to evaluation of genetic diversity of 20 genotypes of weed dodder (Cuscuta epithymum L.) from Golestan and Mazandarn provinces. 6 ISSR primers amplified 90 bands that 76.33% bands were polymorphism. Average polymorphism information content (PIC) and average marker index value was 0.26 and 20 for ISSR markers respectively. RAPD markers amplified 195 bands that more than 95% bands were polymorphism. Average PIC and average MI was 0.27 and 24.7 jcs-cuscuta-epithymum-19688for this markers calculated. Cluster analysis based on DICE coefficient and UPGMA algorithm showed that there is wide variation in samples collected. The average of similarity based on ISSR markers was 0.57 and average of similarity based on RAPD markers was 0.45. This high level of diversity could be related to the existents of several sub species in weed dodder (Cuscuta epithymum L.) and the high discrimination power of molecular markers that be used in this study.

jbes-vol4no4-p19-26Get the original articles in Source: Volume 4, Number 4, April 2014 – JBES

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

Related Post: Organic cultivation of medicinal plants: a review — JBES

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