Rachna Kumari, Maheshwar, P. Trivedi, Birendra Prasad
Microbial and Molecular Genetics Lab, Depatment of Botany, Patna University, Patna-800005, India
Key words: Rauvolfia, Reserpine, Manures, NPK fertilizer, Growth performance, Root yield.
The study revealed the growth performance, root yield and reserpine content in the root part of R. serpentina and R. tetraphylla under four different treatment regimes (T1,T2, T3, T4). Three types of cutting like root, root-stem junction and stem of 2 to 2.5 years old plants of both the species were used. It was concluded that root formation percentage from cuttings of root, root-stem junction and stem under different treatment conditions in R. serpentina were varied from 33.33–85.00 %, 31.67– 50.00% and 20.00–33.33 %, respectively. A similar trend was observed in in R. tetraphylla. Vermicompost (T1) showed comparatively maximum root biomass increased percentage followed by Cowpat (T2) in R. serpentina though it was more or less similar in R. tetraphylla. The application of T1, T2, T3 and T4 compositions significantly (P=0.01) increased root mean dry weight in all plant cutting types of both the species against control and the trend was root-stem junction cutting > root cutting > stem cutting. Application of NPK fertilizer decreased the soil pH from 6.73 (T5) to 6.6 (T3 and T4). In T1 and T2 pots the available soil nitrogen significantly increased by 0.0079 % and 0.0072 %, respectively. NAA at 50 ppm had highest impact on root formation followed by IAA and IBA then at 5 ppm of 2,4–D in R. tetraphylla. Quantification of reserpine in root of treated plants by HPTLC revealed significant variation.
Get the original articles in Source: Volume 6, Number 4, April 2015 – IJAAR
Published By: International Network for Natural Sciences