Sina Ghanbari, Seyed Kamal Kazemitabar, Hamid Najafi Zarini
MSc Student of Plant Breeding, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran
Associated Professor of Plant Biotechnology, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran
Assistant Professor of Plant Breeding, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran
Key words: Sesame (Sesamumindicum L.), Callus, 2,4-D, NAA.
Sesamumindicum L. belongs to family pedaliaceae that these family, including 60 species. Sesame is the most important oil-seed crop of semi-arid tropics and a source of high quality cooking oil and protein. In recent years, plant tissue culture techniques has become a powerful tool for the propagation of many plant species. In this research weight and diameter of callus were analyzed to determination of appropriate medium culture. This study were performed on fall of 2013 in complete randomized design with three replicated. The MS medium culture was contain NAA and 2,4-D hormones. ANOVA statistical analysis showed significant difference at 1% level. Highest callus diameter belong to medium contain 0.2 mg/l 2,4-D and 0.12 mg/l NAA, also maximum callus weight belong to 0.2 mg/l 2,4-D and 0.16 mg/l NAA. Therefore, hormones amount which used in this research can induced callus in sesame.
Get the original articles in Source: Volume 4, Number 3, March 2014 – JBES
Published By: International Network for Natural Sciences