Assessment of enterovirus contamination in vegetable samples in Morocco by reverse transcription Nested-PCR

fruity-vegetablesK.Ibenyassine, Y. Karamoko, M. El fennouni, R. Ait Mhand, M. Elfahim, M. N. Benchekroun, M. M. Ennaji

Laboratory of Virology and Hygiene & Microbiology, Department of Biology, Faculty of Science and Technology, University Hassan II-Mohammedia, Mohammedia, Morocco

National Center for Scientific and Technical Research (CNRST), Technical Platforms-UATRS Division, Rabat, Morocco

Laboratory of Environmental Biotechnology and Health, Department of Biology, Faculty of Science and Technology, University Hassan II Mohammedia- BP 146 20650Mohammedia, Morocco

University of Abobo-Adjamé, UFR SN 02 BP 801 Abidjan, Côte d’Ivoire

Key words: Vegetables, enterovirus, RT-PCR detection, wastewater, Morocco.

Abstract

Vegetables are often implicated in the transmission for food-borne diseases, but local research on enteric viruses in vegetables is scarce despite its importance for public health. In this paper we report the results of an investigation on the presence of enteroviruses in vegetables irrigated by wastewater in Morocco. a8c92ffe625b77a88ca1d83ef3f344d2Viruses were concentrated from vegetable tissues by direct glycine elution and PEG 8000 precipitation. Total RNA was then extracted from the PEG precipitate by the guanidine thiocyanate method and transcribed into cDNA followed by “nested-PCR”. Enterovirus genomes were detected in 16% of analysed samples. The range of positive samples is: eggplant, broad bean, tomato, pepper, potato, garden pea and radish. The Enterovirus contamination observed in this study should be considered as a serious risk to public health.

Get the original articles in Source: Volume 2, Number 7, July 2012 – IJAAR

Published By: International Journal of Agronomy and Agricultural Research (IJAAR)

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