K.Ibenyassine, Y. Karamoko, M. El fennouni, R. Ait Mhand, M. Elfahim, M. N. Benchekroun, M. M. Ennaji
Laboratory of Virology and Hygiene & Microbiology, Department of Biology, Faculty of Science and Technology, University Hassan II-Mohammedia, Mohammedia, Morocco
National Center for Scientific and Technical Research (CNRST), Technical Platforms-UATRS Division, Rabat, Morocco
Laboratory of Environmental Biotechnology and Health, Department of Biology, Faculty of Science and Technology, University Hassan II Mohammedia- BP 146 20650Mohammedia, Morocco
University of Abobo-Adjamé, UFR SN 02 BP 801 Abidjan, Côte d’Ivoire
Key words: Vegetables, enterovirus, RT-PCR detection, wastewater, Morocco.
Vegetables are often implicated in the transmission for food-borne diseases, but local research on enteric viruses in vegetables is scarce despite its importance for public health. In this paper we report the results of an investigation on the presence of enteroviruses in vegetables irrigated by wastewater in Morocco. Viruses were concentrated from vegetable tissues by direct glycine elution and PEG 8000 precipitation. Total RNA was then extracted from the PEG precipitate by the guanidine thiocyanate method and transcribed into cDNA followed by “nested-PCR”. Enterovirus genomes were detected in 16% of analysed samples. The range of positive samples is: eggplant, broad bean, tomato, pepper, potato, garden pea and radish. The Enterovirus contamination observed in this study should be considered as a serious risk to public health.
Get the original articles in Source: Volume 2, Number 7, July 2012 – IJAAR