Rapid in vitro clonal propagation of a hybrid muskmelon (Cucumis melo L.) cultivar from seedling explants

muskmelonHafizur Rahman, M Shahinozzaman, M Rezaul Karim, M Aminul Hoque, M Monzur Hossain, AKM Rafiul Islam

Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi 6205, Bangladesh

Key words: Muskmelon, clonal propagation, seedling explant, leaf node.


An investigation was undertaken to develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation. Best in vitro seed germination was observed in MS medium containing 0.5 mg/l GA3. Two explants excised from axenic seedlings were tested for shoot proliferation and leaf node showed better shoot proliferation than shoot tip explants.

13musk-melonMaximum shoots (8.22±0.23) per culture were produced from leaf node explants cultured on MS medium augmented with 1.0 mg/l BA. Rooting was found best on 0.1 mg/l NAA containing medium. Rooted plantlets were acclimatized gradually and transferred to the field condition and finally, 82 % plantlets were survived well.

Get the original articles in Source: Volume 2, Number 1, January 2012 – IJAAR

Published By: International Journal of Agronomy and Agricultural Research (IJAAR)

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