GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phenolics Content of Wild Hops (Flemingia strobilifera (L.) W. T. Aiton) – IJB

Luck_plant_.Flemingia_strobilifera

By: Jhoan Rhea L. Pizon, Olga M. Nuñeza, Mylene M. Uy, W.T.P.S.K Senarath

  1. Department of Biological Sciences, Mindanao State University- Iligan Institute of Technology (MSU-IIT), Iligan City, Philippines
  2. Department of Chemistry, Mindanao State University- Iligan Institute of Technology (MSU-IIT), Iligan City, Philippines
  3. Department of Botany, University of Sri Jayewardenepura, Nugegoda, Sri Lanka

Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content.Flemingia_strobilifera The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.

Source of this research articles: GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phenolics Content of Wild Hops (Flemingia strobilifera (L.) W. T. Aiton)

gcms-analysis-and-evaluation-of-invitro-antioxidant-potential-and-total-phenolics-content-of-wild-hops-flemingia-strobilifera-l-w-t-aiton-1-638

Related Articles: Significance of Trianthema portulacastrum L. in sustenance of Apis florea colonies during dearth period – IJB

Journal Name: International Journal of Biosciences – IJB

Publication Name: International Network For Natural Sciences (INNSPUB)

DOWNLOAD PDF

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s