Solid state fermentation of Lathyrus sativus and sugarcane bagasse by Pleurotus sajor-caju – IJAAR An Open Access Research Journals


By Md. Rezaul Karim1, Zinat Mahal2, Safia Iqbal1, Harun or Rashid2, M. Abu Hena Mostofa Jamal1, Md. Azizul Islam1, Md. Mafizur Rahman1

  1. Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
  2. Institute of Food and Radiation biology, Atomic Energy Commission, Bangladesh
Lathyrus sativus (Khesari  plant) and sugarcane bagasse  are considered as  agro  wastes. Khesari plants are generally grown in fields as weeds and they have to be removed prior to cultivation. Taking this view in mind, we have investigated  the conversion of these lignocellulosic agro-waste as an enriched feed stock for cattle via by solid state fermentation using a cellulolytic fungus, Pleurotus sajor-caju.


The strain required 8 weeks to complete the fermentation on both the untreated and treated (with alkali, lime and presoaked) substrates at 30°C.Higher amounts of reducing sugar and soluble protein were found in each of the lime treated substrates than untreated substrates. Results also indicated that presoaked substrate contain higher amounts of reducing sugar and soluble protein than unsoaked substrate. Among the substrates, mixed substrate (khesari plant + sugarcane bagasse) was found to accumulate higher amount of sugar, 22.15 mg/g and protein, 22.80 mg/g than those of khesari plant in the  5th week of fermentation. 
The treatments that augmented the level of sugar and protein were also found to enhance the cellobiase, carboxy methyl cellulase and avicelase activity of crude culture extracts. These results suggest that lime treatment and presoaking seem to increase the digestibility of the  substrates by the fungal cellulolytic enzymes. During eight weeks of fermentation, relatively higher cellobiase activity was found as compared to that of carboxymethylcellulase and avicelase at 30o C for the fungul strain. The results of the present study clearly indicate that fungal  conversion with pretreatment transform these lignocellulosic  agro-wastes to a nutritionally enriched animal feed.
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Journal Name: International Journal of Agronomy and Agricultural Research (IJAAR)

Publication Name: International Network For Natural Sciences (INNSPUB)



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