Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.)

Credit: Photo by Aldo De Bastiani

Credit: Photo by Aldo De Bastiani

Seyed Kamal Kazemitabar, Simin Azoush, Amin Sadeghi Alikelayeh
Key words: Genetic diversity, weed dodder (Cuscuta epithymum L), RAPD, ISSR
Abstract

Weed dodder is a perennial herb belonging to the Convolvulaceae family and is widely used as a valuable herbal drug in traditional medicine. In this study, ISSR and RAPD markers were used to evaluation of genetic diversity of 20 genotypes of weed dodder (Cuscuta epithymum L.) from Golestan and Mazandarn provinces. 6 ISSR primers amplified 90 bands that 76.33% bands were polymorphism. Average polymorphism information content (PIC) and average marker index value was 0.26 and 20 for ISSR markers respectively. RAPD markers amplified 195 bands that more than 95% bands were polymorphism. Average PIC and average MI was 0.27 and 24.7 for this markers calculated. Cluster analysis based on DICE coefficient and UPGMA algorithm showed that there is wide variation in samples collected. The average of similarity based on ISSR markers was 0.57 and average of similarity based on RAPD markers was 0.45. This high level of diversity could be related to the existents of several sub species in weed dodder (Cuscuta epithymum L.) and the high discrimination power of molecular markers that be used in this study.
Full Article Available: Volume 4, Number 4, April 2014 – JBES
Published on: Journal of Biodiversity and Environmental Sciences (JBES)

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