Archive | May 2015

Characterization of the dual activity of an endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier against carboxymethylcellulose and xylan

Macrotermes subhyalinus

J.B. Fagbohoun, Y. Karamoko, S. Dabonné, E.J.P. Kouadio, E.A. Due, L. P. Kouamé

Key words: Bifunctional enzyme, carboxymethylcellulase, endo-beta-D-glycosidase, termite little soldier, Macrotermes subhyalinus, xylanase.

Abstract

This work reports the characterization of endo-beta-D-glycosidase from salivary gland of little soldier of Macrotermes subhyalinus. Based on thin-layer chromatographic analysis of the degradation products, the carboxymethylcellulase activity produced glucose, cellobiose and cellodextrins from carboxymethylcellulose as the substrate. When xylan from Birchwood was used, end products were xylobiose and xylodextrins. The presence of metal ions such as NaCl, MgCl2 and NH4Cl positively influenced the activity of β-glucosidase but the activity was inhibited in presence of CuCl2, ZnCl2, SDS.

Full Article Available here: Volume 2, Number 3, November 2012 – IJBB

Published on: International Journal of Biomolecules and Biomedicine (IJBB)

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In vivo evaluation of antidiarrhoeal activity of ethanolic extract of leaf and bark of Ficus carica Linn.

Ficus carica Linn

Ashraf-ul Kabir, Ninadh Malrina D’Costa, Mehdi Bin Samad, JMA Hannan

Key words: Antidiarrhoeal, castor oil, enteropooling, Ficus carica Linn, prostaglandin E2.

Abstract

Ficus carica Linn is occasionally used in preparation of local traditional medicines used in the treatment of diarrhoea in Bangladesh. Our present studies make an attempt toward validating this traditional use by investigating antidiarrhoeal activity of F. carica Linn. Continue reading

The impact of nano Fe-chelate, Fe-EDDHA Non-nano and FeSO4 on the growth and physiological index in lettuce (Lactuca sativa L.) varieties grown in NFT

Lactuca sativa L

Musa Jalali, Mehri Zargani

Key words: Chlorophyll a, chlorophyll b, Nano Fe-chelate, lettuce, Fe.

Abstract

It is one of the micronutrients Fe, there is enough for plant growth and chlorophyll formation it is necessary. Element for many physiological processes, including the manufacture of chlorophyll and the biochemical reactions of oxidation and reduction, photosynthesis, respiration and enzyme systems is essential. Continue reading

Screening of Gossypium hirsutum germplasm against Verticillium dahlia Kleb by natural infection

Verticillium wilt

Blessing Chapepa, James Chitamba, Pepukai Manjeru, Nhamo Mudada

Key words: Verticillium wilt; Gossypium hirsutum, germplasm.

Abstract

An experiment on screening of cotton germplasm against Verticillium wilt caused by Verticillium dahlia Kleb was carried out at Cotton Research Institute in 2008, 2009 and 2010 seasons in Zimbabwe. The objective of the study was to identify cotton germplasm with considerable resistance to the disease under natural infection for use in cotton variety development programmes. Continue reading

Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.)

Credit: Photo by Aldo De Bastiani

Credit: Photo by Aldo De Bastiani

Seyed Kamal Kazemitabar, Simin Azoush, Amin Sadeghi Alikelayeh
Key words: Genetic diversity, weed dodder (Cuscuta epithymum L), RAPD, ISSR
Abstract

Weed dodder is a perennial herb belonging to the Convolvulaceae family and is widely used as a valuable herbal drug in traditional medicine. In this study, ISSR and RAPD markers were used to evaluation of genetic diversity of 20 genotypes of weed dodder (Cuscuta epithymum L.) from Golestan and Mazandarn provinces. Continue reading